Journal: Cell & Bioscience

Article Title: DNA methylation-mediated silencing of MEOX1 promotes glycolysis and immune evasion in colorectal cancer cells through inhibition of GLP2R transcription

doi: 10.1186/s13578-026-01534-x

Figure Lengend Snippet: GLP2R modulates Hippo signaling and glycolysis in CRC cells. A The impact of GLP2R overexpression on the extent of MST1/MST2, LATS1/LATS2, and YAP1 phosphorylation in CRC cells by western blot assay. B The effect of GLP2R overexpression on YAP1 protein expression in different cell fractions by western blot assay. C The impact of GLP2R overexpression on glycolytic activity and glycolytic reserve in CRC cells was examined using ECAR. D The effects of GLP2R overexpression on mitochondrial respiration in CRC cells were examined using OCR. E The impact of GLP2R overexpression on the glucose uptake capacity of CRC cells was evaluated using fluorescent probes. F The impact of GLP2R overexpression on lactate release in CRC cells was evaluated using the lactate assay kit. G The impact of GLP2R overexpression on the expression of glycolytic enzymes GLUT1, LDHA, and PKM in CRC cells was detected by RT-qPCR. H Immunohistochemical assessment of YAP1 and glycolytic enzyme expression in tumor tissues and adjacent tissues of CRC patients (n = 35). I Pearson’s correlation analysis between GLP2R, YAP1, and glycolytic enzyme expression in CRC tumor tissues (n = 35). J Pearson’s correlation analysis between YAP1 and glycolytic enzyme expression in CRC tumor tissues (n = 35). Data are presented as means ± SEM of n = 5 C – G or n = 3 A-B independent biological replicates. Statistical analyses were performed using an unpaired t-test E – F , paired t-test H , or two-way ANOVA A-D, G

Article Snippet: The membrane was blocked in 5% BSA at RT for 1 h, and incubated with the primary antibody: GLP2R (1:2000, A22049, ABclonal), p-LATS1 (phospho T1079)/LATS2 (phospho T1041) (1:1000, AP1517, ABclonal), p-MST1 (phospho T183)/MST2 (phospho T180) (1:1000, ab79199, Abcam), p-YAP1 (phospho S127) (1:500, AP0489, ABclonal), YAP1 (1:10,000, A19134, ABclonal), β-actin (1:5000, AF5003, Beyotime), and Histone H3 (1:2000, AG0175, Beyotime) at 4 °C overnight and subsequent incubation with the HRP-coupled secondary antibody for 1 h at RT.

Techniques: Over Expression, Phospho-proteomics, Western Blot, Expressing, Activity Assay, Lactate Assay, Quantitative RT-PCR, Immunohistochemical staining

Journal: Cell & Bioscience

Article Title: DNA methylation-mediated silencing of MEOX1 promotes glycolysis and immune evasion in colorectal cancer cells through inhibition of GLP2R transcription

doi: 10.1186/s13578-026-01534-x

Figure Lengend Snippet: The suppressing effects of GLP2R on CRC cell malignant behavior are dependent on blocking the YAP1-mediated glycolytic pathway. A The effect of the MST1/2 inhibitor XMU-MP-1 on the extent of MST1/MST2, LATS1/LATS2, and YAP1 phosphorylation and total protein of YAP1 in CRC cells by western blot assay. B The effect of XMU-MP-1 on glycolytic activity and glycolytic reserve in CRC cells was examined using ECAR. C The effect of XMU-MP-1 on the glucose uptake capacity of CRC cells was examined using fluorescent probes. D The impact of XMU-MP-1 on lactate release in CRC cells was examined using the lactate assay kit. E The impact of XMU-MP-1 on the proliferation of CRC cells was examined using colony formation assays. The impact of XMU-MP-1 on the migration and invasion of CRC cells was assessed by wound healing assay F and matrix gel invasion assay G . H The proliferative activity of CD4 + T cells was assessed by flow cytometry analysis. I HLA-DR expression on the surface of MoDC cells was assessed by flow cytometry analysis. Data are presented as means ± SEM of n = 5 B-I or n = 3 A independent biological replicates. Statistical analyses were performed using an unpaired t-test C – I or two-way ANOVA ( A - B )

Article Snippet: The membrane was blocked in 5% BSA at RT for 1 h, and incubated with the primary antibody: GLP2R (1:2000, A22049, ABclonal), p-LATS1 (phospho T1079)/LATS2 (phospho T1041) (1:1000, AP1517, ABclonal), p-MST1 (phospho T183)/MST2 (phospho T180) (1:1000, ab79199, Abcam), p-YAP1 (phospho S127) (1:500, AP0489, ABclonal), YAP1 (1:10,000, A19134, ABclonal), β-actin (1:5000, AF5003, Beyotime), and Histone H3 (1:2000, AG0175, Beyotime) at 4 °C overnight and subsequent incubation with the HRP-coupled secondary antibody for 1 h at RT.

Techniques: Blocking Assay, Phospho-proteomics, Western Blot, Activity Assay, Lactate Assay, Migration, Wound Healing Assay, Invasion Assay, Flow Cytometry, Expressing

Journal: Cardiovascular Diabetology

Article Title: Cardioprotective mechanism of ω-3 fatty acid icosapent ethyl (IPE) in cardiomyocytes: role in high glucose and shear stress-induced mechano-transduction dysregulation

doi: 10.1186/s12933-025-03033-8

Figure Lengend Snippet: IPE effects on mechano-transduction in AC16 cells exposed to hyperglycemia. Western blot analysis for p-MST1 ( A ), p-LATS1 ( B ), active YAP ( C ) and TAZ ( D ) in AC16 cells exposed to NG concentration (NG), cells exposed to high glucose concentration (HG), and cells co-treated with HG and 40 µM IPE (HG + IPE). The histograms show the densitometric analysis of 3 separate experiments representing the relative expression of proteins; NG value was set as 1. Data are mean ± SEM. * P < 0.05 versus NG; ** P < 0.05 versus HG 7 days

Article Snippet: Membranes were blocked with 5% milk in TBS-T (Tween 20 buffered with 0.15% Tris pH 8) at room temperature for 1 h and then incubated with the following primary antibodies diluted in TBS-T: p-MST1 (1:1000) (#49,332, Cell signaling Technology), p-LATS1 (1:1000) (#8654, Cell signaling Technology), active YAP (1:1000) (#ab205270, Abcam), TAZ (1:1000) (#83,669, Cell signaling Technology), Oxidative stress defense western blot cocktail (1:1000) (# ab179843 Catalase, SOD1, TRX, Abcam), p-NF-kB (1:1000) (E-AB-68092, ELAB Science), IL-6 (1:1000) (E-AB-30095, Elabscience), SOD2 (1:1000) (#ab13533, Abcam), p-AMPK (1:1000) (E-AB-21121, ELAB Science), PPAR-α (1:1000) (#ab227074, Abcam), PPAR-γ (1:1000) (#2443, Cell signaling Technology), Integrin β3 (1:1000) (#13,166, Cell signaling Technology), Bcl-2 (1:1000) (E-AB-15522, ELAB Science), Bax (1:1000) (E-AB-22128, ELAB Science) overnight at 4°C.

Techniques: Transduction, Western Blot, Concentration Assay, Expressing

Journal: Cardiovascular Diabetology

Article Title: Cardioprotective mechanism of ω-3 fatty acid icosapent ethyl (IPE) in cardiomyocytes: role in high glucose and shear stress-induced mechano-transduction dysregulation

doi: 10.1186/s12933-025-03033-8

Figure Lengend Snippet: Effects of IPE on mechano-transduction in a dynamic shear stress model of cardiomyocytes. Western blot for p-MST1 ( A ), p-LATS1 ( B ), YAP ( C ), TAZ ( F ), Integrin β3 ( G ), in cardiomyocytes AC16 in static normal glucose condition (NG), cells exposed to turbulent flow in normal glucose condition (NG FLOW) and cells exposed to turbulent flow in normal glucose condition in presence of IPE (NG FLOW + IPE). The histograms show the densitometric analysis of 3 separate experiments representing the relative expression; NG value was set as 1. * P < 0.05 vs NG; ** P < 0.05 vs NG FLOW . D Immunofluorescence staining for active YAP (green) and nuclei (blue) in Ac16 in NG, NG FLOW, NG FLOW + IPE. Merge columns images show overlapping signals. Scale bar = 100 µm; E) YAP intensity ratio (nuclear/cytoplasmic) from cells randomly selected from 3 independent experiments. * P < 0.05 vs. NG; ** P < 0.05 vs NG FLOW

Article Snippet: Membranes were blocked with 5% milk in TBS-T (Tween 20 buffered with 0.15% Tris pH 8) at room temperature for 1 h and then incubated with the following primary antibodies diluted in TBS-T: p-MST1 (1:1000) (#49,332, Cell signaling Technology), p-LATS1 (1:1000) (#8654, Cell signaling Technology), active YAP (1:1000) (#ab205270, Abcam), TAZ (1:1000) (#83,669, Cell signaling Technology), Oxidative stress defense western blot cocktail (1:1000) (# ab179843 Catalase, SOD1, TRX, Abcam), p-NF-kB (1:1000) (E-AB-68092, ELAB Science), IL-6 (1:1000) (E-AB-30095, Elabscience), SOD2 (1:1000) (#ab13533, Abcam), p-AMPK (1:1000) (E-AB-21121, ELAB Science), PPAR-α (1:1000) (#ab227074, Abcam), PPAR-γ (1:1000) (#2443, Cell signaling Technology), Integrin β3 (1:1000) (#13,166, Cell signaling Technology), Bcl-2 (1:1000) (E-AB-15522, ELAB Science), Bax (1:1000) (E-AB-22128, ELAB Science) overnight at 4°C.

Techniques: Transduction, Shear, Western Blot, Expressing, Immunofluorescence, Staining